This has been facilitated by a shared library of plasmids that allows for the Gateway method of restriction-free, modular assembly of driver/transgene combinations (Roure et al. 2007).
Furthermore, determination of the true boundaries of direct repeats by traditional methods of restriction mapping and direct sequencing is laborious and time consuming [ 47].
We then decided to develop a novel cloning method independent of restriction site and ligase.
Promising molecular based applications are also viable alternatives such as fluorescent in situ hydridization (FISH) of group-specific oligonucleotide probes or the high-throughput method of terminal restriction fragment length polymorphism (T-RFLP) used to track specific populations through space and time.
Since BDR therefore reduces reproduction and feeding rates while robustly extending lifespan, it is a method of dietary restriction that successfully recapitulates many aspects of mammalian DR (Table 1).
24 In this study, one principle of CARD was to adopt the proper method of carbohydrate restriction according to a patient's baseline HbA1c level.
The method of food restriction was intended to produce similar weight reductions in both sexes, but actual mean weight loss was 15% in males and 10% in females.
In this study, we employed a grain legume crop Lupinus angustifolius (lupin) as a test case, and examined the utility of an NGS-based method of RAD (restriction-site associated DNA) sequencing as DNA fingerprinting for rapid, cost-effective marker development tagging a disease resistance gene for molecular breeding.
Additionally, we took advantage of a high-throughput genotyping-by-sequencing method, wherein thousands of restriction site associated DNA (RAD) tags are sequenced, aligned, and mined for SNPs.
The RRBS method makes use of restriction enzyme digestion to selectively analyze genomic regions enriched for CpG sites in a methylation-independent manner, thus achieving a high coverage of CpG rich regions while greatly reducing sequencing read requirement.
