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The present study describes a novel, computer-assisted video image analysis method of quantitating immunostained cells within tissue sections, providing continuous graphical data.
These results were also supported using the secondary method of quantitating vascular leakage into tissues by measuring EBD leakage into tissues.
Digital genome scanning (DGS) is a method of quantitating gene copy number by enumerating short genomic DNA fragments (termed real tags) that are generated experimentally by MboI endonuclease digestion.
This method of quantitating serum samples against plasma calibration also met the FDA ± 15% method precision requirement for all target analytes and also met the FDA ± 15% method accuracy requirement for PFOS, M556, and M570.
Methods for studying absorption are categorized according to the technique for administering the test substance such as inclusion in the diet or by gastric and intestinal placement and the method of quantitating the degree of absorption such as determining the appearance of a test substance in systemic fluids or its disappearance from its site of administration in the intestine.
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Two different methods of quantitating RAP gave comparable results.> -wrap-foot> alignmentent of the human TEN-domain with the Tetrahymena thermophila TEN-domain in combination with a secondary structure prediction shows that K78 and R132 are in close proximity to each other, on a surface distinct from the proposed DNA-binding region of the TEN-domain.
Development of methods of quantitating C. pneumoniae in whole blood and PBMC have been developed using TaqMan or LightCycler real-time PCR detection technologies, and Berger et al used a quantitative assay for the detection of C. pneumoniae DNA in PBMC and atherosclerotic plaque [ 21].
However, the use of the dot blot method for quantitating multiple different constructs (for example containing different truncations of a single protein when annotated domain boundary information is absent) could be very useful and the pre-screen and dot blot combination is useful for optimising culture conditions for a single protein.
There are a variety of methods for quantitating viruses.
Recently, using an ultrasensitive method for quantitating methods, ctDNA was detected in 100% of patients with stage II IV NSCLC and in 50% of patients with stage I, with 96% specificity for mutant allele fractions down to ~0.02% [ 88].
DOI: http://dx.doi.org/10.7554/eLife.02040.011 10.7554/eLiFigure40.012 figure 2—figure supplement 3. Comparison of image analysis methods for quantitating cdr2p fluorescence in the nodes.
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