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These results demonstrated that the introduction method of promoters and iron species plays a crucial role in the design and fabrication of highly active, selective and stable iron-based composite catalysts for the FTO reaction.
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We used two methods of promoter extraction.
In the case of MMS, it had circumvented the possibility of regulatory inspection by marketing itself as a "water purifier" – a valid description of the its chemical nature, but not its intended application, leading the Irish Health Productions Regulatory Authority to describe the methods of MMS promoters as "underground and unorthodox".
However, since there is generally limited data on known promoter locations and/or unreliable prediction methods, the identification of promoters is often difficult and makes their use problematic for operon prediction.
We performed a statistical analysis (see Methods) of the promoter sequences (defined as the 1000-bp upstregionsgiofs) of ABA-responsive genes in guard cells and leaves, to check whether the known motifs would be enriched in our gene sets.
To highlight this shortcoming, we formulate and evaluate several methods of quantifying promoter-localised DNA methylation and demonstrate that its naïve integration into previous models is unable to improve prediction accuracy.
MSP-PCR is a well-established, straightforward and rapid PCR-based method for analysis of promoter methylation and gene silencing.
Danner and Soppa (8) utilized trimethoprim resistance as a method for classification of promoter strength by culturing a randomly selected set of mutants in growth medium supplemented with various dilutions of the drug.
Single (Ba) and double promoted (Ba and Cs) Ru catalysts supported on calcined Mg Al hydrotalcite were prepared by polyol reduction method to investigate the role of promoters on catalytic activity for NH3 synthesis at atmospheric pressure.
The present method optimizes the insertion procedure of promoters selected from a previously characterized library.
Second, we did not conduct a systematic approach of all CpG sites in the 21 genes but focused on qPCR method of CpG islands in promoter regions.
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