Thus, alternative methods of proliferation assessment are of interest, in particular if they could be used in routine practice.
As discrepancies are common between these two methods of proliferation evaluation, [ 42] a parallel cannot be drawn between this study and our results on this variable.
For these reasons the development of non-invasive, reproducible and validated methods of proliferation measurement will be a major advance for the evaluation of anti-neoplastic agents and for identifying non-responders early in their treatment so that they can be offered alternative and possibly more efficacious therapies.
Consequently, this study was conducted on a large, well-characterized series of early (stage I through III) invasive BC with long-term follow-up to study the prognostic value of MS and Ki-67LI, as methods of proliferation assays, in different BC molecular classes.
Therefore, the aim of this study was to examine whether hypoxia can influence stem-like properties of ovarian cancer cell lines in vitro with the methods of proliferation assay, cell cycle analysis, infiltration assay, colony formation assay, sphere formation assay, SP, FACS and Western blotting.
Rather than identifying cells engaged in particular phases of the growth cycle, an alternative method of assessing proliferation is to detect antigens that are closely associated with proliferation using immunohistochemistry.
MIB-1 immunostaining can be used as an effective method of assessing proliferation in human breast carcinomas.
Recently, Misell [ 88] and colleagues reported on a new method of measuring proliferation in vivo using heavy water labelling followed by mass spectrometry analysis.
The best documented method of measuring proliferation in breast cancer is SPF (S-phase fraction) assessed by flow-cytometry (Sigurdsson et al, 1990).
The discrepancy of results may relate to the fact that we used a more sensitive method for measurement of proliferation, or to phenothypic changes that may have occurred during cultivating of cells for years in vitro.
