Sentence examples for method of primer from inspiring English sources

As a proof-of-principle demonstration, the method of primer design was applied in colony PCR for identifying plasmid DNA deletion or insertion mutants after site-directed mutagenesis.

Moreover, the method of primer design around the centromeric region was discussed, which would provide insight into fine mapping of the functional genes in plant centromeres.

All transcription start sites are probably not used with the same frequency and it is reasonable to propose that those detected by the not very sensitive method of primer extension are used most often.

The full length of Glu-1 ORFs was obtained by using the method of primer walking and nested deletion.

Plasmid DNA of the colony with the ability to cleave 5-bromo-4-chloro-3-indolyl α- l-fucopyranoside (X-Fuc) was isolated and sequenced by the method of primer walking (Geneart).

Full-length sequence was obtained by using a previously described method of primer walking and the 5′/3′ Rapid Amplification of cDNA Ends (RACE) Kit (Roche), which was used to determine the sequence of the segment ends (5 ).

Although various methods of primer labeling and SSR allele detection in eucalypts was reported (Ottewell et al. [2005]; Missiaggia and Grattapaglia [2006]; Faria et al. [2011]; Subashini et al. [2013]), difficulties in accuracy of allele sizing continue to exist.

We recognize that the methods section of Primer Notes is particularly vulnerable to self-plagiarism; after all, there are only so many different ways to describe specific steps of a procedure, such as thermocycler running conditions or PCR reactions.

In contrast to this method of designing primers, the original 'manos' MY09/11 primers used in previous prevalence studies were 'degenerate', which meant they did not necessarily provide reproducible estimates of HPV types detectable in samples [ 20].

In the present work, we propose a rational method of designing primers in order to amplify the variable region of heavy chain (VH) and variable region of light chain (VL) domains for framework 1 (FR1) of immunoglobulins.

As the sample sizes from these studies were too small to further stratify by methylation method or location of primer, we analysed the pooled specificity of these four studies.