Referring to the Release 10 scheme[8], to avoid border vagueness, we can consider the method of overlapping among subsets, and the overlapping ratio between adjacent subsets is 1/2.
The underlying forward model was computed using the method of overlapping spheres as described by Huang, Mosher, and Leahy (1999).
Moreover, the records were double-checked by a second laboratory (Biosignal Analysis and Medical Imaging Laboratory at University of Eastern Finland, Kuopio, Finland) by calculating the Fourier-based spectrum with the Welch's averaged periodogram method (length of overlapping epochs was 1000 ms and overlap was 75%).
Although CTCF binding can vary among cell types [ 25], when we define #CTCF using joint CTCF ChIP-seq peaks (see Methods) instead of overlapping CTCF peaks, the results did not change (Table S1 in Additional file 1).> -wrap-foot> a " d", intergenic distance; " #CTCF", number of overlapping CTCF-binding sites; " ΔCpG O/E", difference in upstream DNA methylation.
Employing the previously described method of concatenating overlapping conserved syntenic segments [ 34], the eutherian mammal genome data permitted the seamless assembly of conserved segments into ancestral chromosomes.
When there are only a few users, the performance of non-overlapping method is not better than that of overlapping method.
After presenting an overview of such method and of overlap detection in systems of hard spherocylinders, the application of the method for granular systems is discussed.
Thresholds for each of the methods and functions of overlapping quality are defined.
Synthesis of the modified SAG1 ORF (residues 77 to 322, excluding the signal peptide in the N-terminus and the 14 hydrophobic amino acids in the C-terminus) was carried out by the overlapping extension PCR method using the series of overlapping oligos shown in Table 2.
While with the growth of user number and successful paired rate, the advantage of overlapping method becomes gradually significant.
