The Nei [ 62] minimum genetic distance method is applicable to any population without regard to the number of alleles per locus, the pattern of evolutionary forces and the reproductive method of the organism studied.
The modern method of classifying organisms was devised by Swedish biologist Carl von Linné, better known by his Latin name Carolus Linnaeus (1707 78).
A bacterial culture is a method of bacteria organisms by allowing them to reproduce in predetermined culture media under controlled laboratory conditions.
The development of EST databases has been recognized as a rapid method of sampling an organism's transcriptome and is complementary to a whole genome-sequencing project [ 46].
Apart from a few species for which a complete genome sequence is already available, gene discovery in most plants is primarily based on the sample sequencing of expressed sequence tags (ESTs) and the development of EST databases has been recognized as a rapid method of sampling an organism's transcriptome.
On the other hand, the commercial immunochromatography kit only detected antigens from 18 hours enrichment culture because the method requires 10 CFU/mL of organism (Tables 4 and 6).
Because the gene targeting deletion method of non-model organisms including Brassica has not been developed, it has been difficult to examine the necessity and roles of the identified candidates in self-incompatibility mechanism in Brassica.
The molecular identification of marine nematodes is rapidly advancing and already uses second generation sequencing techniques to overcome the taxonomic hurdles and time consuming extraction methods of meiofaunal organisms [12], [36].
This may be related to improvements in isolating and culturing methods of anaerobic organisms from the oral cavity.
In future work, we will try to integrate more data samples and more critical time points of data, and design new methods of model organisms to unearth more deeply the mechanisms and processes.
