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An alternative method of normalising clone selection, which has been used by others, is by reassociation of single-stranded library plasmids at relatively low Cot to remove highly expressed clones [ 22, 23].
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In contrast, microarray analysis assesses the transcript abundance of tens of thousands of genes, thus it has required the application of statistical methods to normalise the distribution of signals and also requires correction for large samples sets, so called false discovery rate correction [ 3, 4].
The method also builds upon a calculation of normalising the number of neighbours of a given domain by its frequency.
This has the effect of normalising their behaviour.
Just as this may normalise the devastation of opencast mining, what BP is buying with its £10m is a way of normalising its extraordinarily destructive activities.
There are a paucity of studies investigating the clinical significance of normalising angiogenic axis.
One of the main questions to address is effectively the relevance of the use of HKGs as an appropriate method to normalise RT QPCR results.
Levels of expression were evaluated using the absolute quantification method normalising against the housekeeping gene GAPDH (ABI technical bulletin 2, www.appliedbiosystems.com).
The relative expressions of caspase-3, 7, 8, and 9 were calculated using the ΔΔ-Ct method, normalising to β-actin.
Expression ratios of test samples relative to reference were calculated using the comparative Ct method (Pfaffl, 2001), normalising to four different housekeeping genes.
Quantification was assessed by the comparative Δ CT method normalising CT values to β-actin.
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