Sentence examples for method of mutagenesis from inspiring English sources

A new biotechnological method of mutagenesis, called activation tagging mutagenesis (ATM), has increased the understanding of development in various plants by intensifying the range of gene expressions and accumulation of secondary metabolites.

Consistent with the EMS method of mutagenesis, these are all point mutations that result in a single amino acid substitution in the ATPalpha protein.

Although in vitro cloning approaches based on the use of high-fidelity polymerases for PCR amplification have significantly improved in recent years, the use of in vivo approaches should allow a more accurate method of mutagenesis due to the use of the cells own high-fidelity replication system which includes proof reading.

To create the His-tagged free-Ig domain of β3 (β3-Ig-6His; Fig. 1 B), nucleotides translating to residues T130-E191 of the mature human protein, and cloned into the plasmid pFastBacMam, were deleted, and the ECD nucleotides were fused to a C-terminal 6His tag using the QuickChange method of mutagenesis (Stratagene, Cambridge, UK).

Additional differences could be related to technical differences in two screens including the methods of mutagenesis (insertion duplication vs. transposon insertion) and mutant detection (PCR vs. Southern hybridization).

In contrast to traditional methods of mutagenesis, injection of morpholino oligonucleotides permits much more rapid targeted gene knockdown.

These methods involve introduction of diversity into the antibody genes by various methods of mutagenesis such as error-prone PCR, DNA or chain shuffling, or oligonucleotide-directed PCR, while affinity selection of the variants with decreasing amounts of antigens [ 16].

An application of 3NPs method to mutagenesis of antibodies [ 27] or coiled-coils [ 30] gave good results.

Application of degenerate oligonucleotides is the straightforward method of saturation mutagenesis.

We will show that this method of fractionation mutagenesis comprises a natural "deletion machine" 84% of the time in the posthexaploidy Brassica lineage and deletion mutations are certainly loss-of-function.

We have described here a simple and facile method for mutagenesis and manipulation of mycobacteriophage genomes.