The traditional method of cooking maize with lime to make tortillas at the rural level is both time-consuming (about 14 to 15 hours) and hard work.
Rather than supporting the identification of Zea spp., this study provides evidence to indicate a lack of reliability and/or credibility of this method for maize identification in archaeological contexts and calls into question the procedures advocated by the author and her associates.
Finding a new method for reproduction of maize hybrid strain is necessary.
The aim of this study is to find a new method of reproduction of maize hybrid strain using engineering restorer lines and engineering sterility lines by gene engineering technology.
Bedolla et al. (1983) tested various methods of cooking maize and sorghum as well as mixtures of the two grains.
A literature review and the descriptive epidemiology investigation allowed development of hypotheses about the relationship between aflatoxicosis and methods of handling maize.
The dynamic counting method ensures comparable results of maize pollen counting; the detection limit remains in the range of <0.1% of the total pollen count, relatively and absolutely, until the maize pollen count is greater than 3 at least.
Therefore, in this study we searched for alternatives methods for seed inoculation of maize and wheat, aiming to avoid the direct contact of bacteria with pesticides.
We applied a new method of in situ measurement of maize pollen deposition described by Hofmann et al. [15] (see Additional file 1: Figure S8).
Another method to confirm proper annotation of maize genes is to examine the syntenous region of the Sorghum genome for an orthologous gene.
