Here, we focus on the sequence-based methods of ligand-binding sites prediction.
Seven different examples were selected for assessing the capacity of the method of detecting ligand-based relationships and to demonstrate optimal search influencing factors.
Here we focus on the method of receptor-ligand docking and scoring, which can be used when a three-dimensional structure of the target is available.
Biopanning with homologous coating antigens on solid plates is a common method of selecting ligands by competition for the small-molecule target.
We focused on the development of methods for ligand immobilization, selection via affinity chromatography, and nucleic acid quantification that can be performed with common laboratory equipment.
In this method, NMR signals of ligand protons are detected if the ligand is bound to the protein.
Figure 1 depicts these different methods schematically. Figure 1 Comparison of the different methods of modifying ligand coverage on CdSe quantum dots where B, S, F, and OR refer to binding, spacer, functional, and organic oligomer groups, respectively.
There are various methods for coupling of ligand molecule on drug delivery system so that drug-carrier system can be targeted to liver cell via receptor ligand processes.
In these methods, increasing concentrations of ligand added to the drug targets studied (in whole cell lysates) prevent the drug targets from binding to the immobilized ligand and thus preclude them from subsequent MS-based detection.
Different methods for coupling of ligand molecule on drug delivery system are coupling of targeting moieties on preformed nanocarriers, coupling of targeting moieties by the post-insertion method, coupling of targeting moieties by the Avidin/Biotin complex, coupling of targeting moieties before nanocarriers formulation.
