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The method of inoculation varies among studies in MycoDB and can include the addition of spores, roots, mycelia, pot culture, field soil, or any combination thereof.
The design of percutaneous devices, susceptibility of various animal species, bacterial strains, amounts of bacteria, method of inoculation and methods for subsequent evaluation of the infection are discussed in detail.
Onesimus, an enslaved African, describes to Cotton Mather the African method of inoculation against smallpox.
The bite had drawn blood and this method of inoculation was presumed to be the route of transmission of the virus.
However, in the present study, no considerable influence of inoculum size or method of inoculation were observed on N. intermedia pellet formation.
The method of inoculation was the same as described above, and three panicles per line or cultivar were inoculated for each time point.
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Treatments consisted of combinations of varying doses of N fertilizer (100 % and 75%% N) and inoculants (1× and 2.5×), and different methods of inoculation (SI, IPF, ILS, and ISS).
The methods of inoculation and pre-culture followed those described in the literatures [17],[17].
Alternative methods of inoculation may increase the utilization of such bacteria in the field and help reduce agricultural costs.
Our results have identified alternative methods of inoculation that were as effective as the standard seed inoculation that may represent an important strategy to avoid the incompatibility between inoculant bacteria and pesticides employed for seed treatment.
In our study we have identified alternative methods of inoculation to avoid the contact of Azospirillum with pesticides applied to the seeds, with an emphasis on leaf spray at the beginning of the vegetative phase.
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