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This method of fragmentation provides more reproducible coverage because all the ions are fragmented each run, and the identified peptides are not limited by which precursor ions are select for tandem MS. Furthermore, since only one fragmented ion scan is performed for each survey scan, there is far more quantitative data for the parent ions.
In order to give a quantitative description for the distribution of fragments, a fractal analysis of damage accumulation is made, and a fractal prediction method of fragmentation resulting from rock blasting is presented.
Harking back to the Dadaist method of fragmentation, Ms. Rainer chose a collage structure to contain the selections.
Our study illustrated that the sensitive UPLC Q-TOF analytical system combined with the MSE method of fragmentation data collection and natUPLC Q-TOFct danalyticalallowsystemlatively rapid and reasonable investigation of the reported and unknown combineds in this complex TCM sample T. asiatica.
Although the most common method of fragmentation, CID, is very useful for the sequencing of O-phosphorylated peptides, its application to N-phosphorylation is limited as a consequence of considerable losses of the phosphate group HPO3− from the phosphoramidate bond even at relatively low collision energy conditions.
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The most appropriate approach for the top-down MS/MS analysis of intact nitrated proteins seems to be a combination of different methods of fragmentation following the reduction and alkylation of any disulfide bonds present.
Hydrodynamic shearing is a method of DNA fragmentation that involves injecting the sample solution through a narrow diameter orifice at high velocity.
A higher order Laplacian model is proposed for enhancement and stabilization of pressure calculation by the MPS (Koshizuka S, Oka Y. Moving particle semi-implicit method for fragmentation of incompressible fluid. Nucl Sci Eng 1996 123 421 34) method.
Presented here is a comparison of 193 nm ultraviolet photodissociation to collision-based methods for fragmentation of protein ions in a high-throughput format using the Escherichia coli ribosome and the fractionated Saccharomyces cerevisiae proteome as models.
In spite of the fact that this method of in source fragmentation is less selective than tandem mass spectrometry, as all ions in the source will be fragmented simultaneously, it has been used by several research groups [10 19].
Several different methods have been reported for in vitro recombination or DNA shuffling: for example, the original Stemmer method of DNase I fragmentation and reassembly, the staggered extension process (STEP), and random priming recombination.
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