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As a control, we used the method of enzyme immobilization in glutaraldehyde vapour (without zeolites).
The advantage of applied method of enzyme immobilization with respect to other techniques is demonstrated.
The developed method of enzyme adsorption on silicalite was compared with the common immobilization procedure in glutaraldehyde vapor.
Thus, the method of enzyme immobilization using PEI/GA/Sil composition is highly effective and perspective for biosensor creation.
In our work, we proposed a method of enzyme adsorption on zeolite monolayers for the creation of potentiometric biosensor.
The method of enzyme adsorption on zeolites can be characterized by advantages such as quickness, simplicity, the absence of toxic chemical reagents, and good reproducibility.
Similar(36)
The method of enzyme-linked immunosorbent assay (ELISA) was used to test serum levels of CRP, IL-6, and TNF-α.
MDA and ADMA levels were measured by the method of enzyme-linked immunosorbent assay.
The conventional methods of enzyme immobilization are physical adsorption, covalent binding, cross-linking and enzyme entrapment with polymers.
Thus, the improvement of methods of enzyme immobilization is an actual trend in the biosensor development [2,3].
Further various methods of enzyme stabilization with respect to their use in aqueous and non-aqueous environment have been given.
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