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Here, we first used the method of endogenous LC3 protein monitoring to ask whether or not basal autophagy is altered in models of loss of DJ-1 function.
This method of endogenous photosensitisation of cells has been exploited for photodynamic therapy (PDT).
A method of endogenous profiling of peptides from OA synovial fluid that resulted in identification of 40 proteins was described by Kamphorst et al. in 2007 [ 17].
Although this method of endogenous protein normalization (EPN) and similar approaches have been reported previously in quantitative proteomics [ 7, 19– 22], our approach is unique.
Increasing the dose of NE significantly augments cardiac output by 11 to 17% [ 21], suggesting NE might recruit some blood from the large venous unstressed volume as another method of "endogenous fluid challenge".
Here, we have used a specific screening and in vivo engineering method of endogenous and expressed Hxt transporters in a xylose-fermenting S. cerevisiae strain in order to solve the sequential sugar uptake predicament.
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The next sections review in more detail three-aforementioned promising strategies toward β-cell replacement or restoration that are currently in preclinical studies: derivation of insulin-producing cells from pluripotent stem cells, reprogramming non-β cells into β cells, and methods of promoting endogenous regeneration.
Development of efficient methods for detection of endogenous H2S in living cells and tissues is of considerable significance for better understanding the biological and pathological functions of H2S.
In order to investigate the role of metal accumulation, we needed an efficient method of reducing the endogenous metal load.
To clarify these issues, we have investigated the effects of PDT on rat femoral arteries, using a second-generation photosensitiser, disulphonated aluminium phthalocyanine, and a new method of photosensitisation, using endogenous synthesis of protoporphyrin IX following systemic administration of 5-aminolaevulinic acid (ALA).
One such method measures the release of endogenous enzymes (e.g., lactate dehydrogenase) in the supernatant during the CTL-mediated cytolysis of target cells [4].
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