This might be due to the fact that even though the cells had been lysed by a physical method of cell disruption, most of the carbohydrates remain bonded with protein matrix that precipitates in the middle phase.
NB: The sample layout for C is NOT the same as in A and B. In addition to the experimental results of yield, purity and assay performance, we considered the relative ease of use, cost and adaptability to our preferred method of tissue disruption (Geno/Grinder™ 2000) when selecting methods for stage 2 (Table 3).
In view of the very gentle method of cell disruption by pressure homogenization [33], [34], lysosomes should still be intact after membrane preparation.
Our ability to liberate the CD8αα+ LNDC from the MLN in significant numbers is critically dependent on the method of LN disruption and DC isolation.
The progenitor method of gene disruption methodologies in C. albicans has been based on the so-called "Ura-blaster" method (Fonzi and Irwin, 1993).
Here, these studies were extended by sequencing the chromosomal regions flanking the amphotericin polyketide synthase genes, and by refining the phage KC515 transduction method for disruption and replacement of S. nodosus genes.
Several methods of tissue disruption were tested, and although DNA yield varied with disruption technique, all methods resulted in successful extractions.
Our study was undertaken to retrieve bacteria from biofilms on titanium and steel surfaces using an in vitro model, and to compare different methods of biofilm disruption for subsequent culture.
This method of monitoring telomere disruption is referred to as a TIF response [ 83].
We present a novel method of targeted gene disruption that involves direct injection of recombinant Cas9 protein complexed with guide RNA into the gonad of the nematode Caenorhabditis elegans.
