A method for detaching the biofilm is required to either enumerate or characterize these bacterial communities.
In addition, the method that we chose must pass through a procedure of detaching cells from culture flasks by dispase or trypsin, which is thought to possibly change the composition and configuration of cell surface proteins [31].
We developed a novel method to detach a monolayer of cultured cells with a supporting collagen gel layer from a calcium alginate gel on a porous membrane in a culture dish by treatment with EDTA as a relatively noninvasive method without using protease.
Even now, I have this habit of detaching myself".
Another issue concerns the possibility of detaching.
The BAL method used in the here-presented studies is highly standardized and yields highly reproducible results compared to more rigorous lavage techniques but is obviously not capable of detaching more sessile macrophages of the lung.
Methods: The general principle of management of detached acute osteochondral fractures is reattachment of the fragment by internal fixation, but the opinions on the quality and structure of the healed tissue that will be obtained after treatment is controversial.
Despite their small size, root pieces from seedlings stages of epiphytes (e.g., P. concreta) yielded fungi with our method despite being detached in the field 3 weeks prior.
By contrast to the classic method that uses detached leaves to evaluate cold tolerance [ 15], we used tissue−cultured grape plants to ensure consistency of plant material for investigating the whole−plant level.
1H NMR analysis of the grafted latex and cleaved polymers from the surface demonstrated that accurate determination of the copolymer composition by this method is possible without detaching polymer chains from surface.
