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Selection criteria were the popularity of the individual datasets in the literature and diversity of the data regarding their authors and the method of decoy generation used.
More specifically, distinct signature patterns can be observed when considering the correlations of the individual Amber99 terms with RMSD for all decoy sets employed in this study, and plotting the distribution of these correlations for every method of decoy generation (see Supplementary Material).
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We show that the standard method of decoy-based error modeling fails to account for the error introduced by these highly similar sequences, leading to a significant underestimation of the false discovery rate.
Current methods of decoy formation have primarily been optimized for potential therapeutic applications.
For example, we observe a consistent presence of positive and (often strong) correlations of the bond stretching term with RMSD on the MOULDER decoy set, which is not observed for any of the other methods of decoy generation.
For the non-bonded terms, much of their discriminatory performance is retained after local minimization of the decoys (see Supplementary Material), indicating that there is a genuine undersampling with respect to these terms by specific methods of decoy generation.
Above all, our experiments therefore underline the importance of evaluating scoring methods on decoy sets that are genuinely representative of the application scenario at which the methods are targeted.
There are several attractive advantages of decoy method over the other gene therapeutic approaches [ 32, 33].
Thus, using the decoy search method of determining FDR this "near zero" FDR would be propagated to modified peptides since the reverse sequence would not produce USTags except in rare cases described above.
When unauthorized access is suspected and then verified, the method uses disinformation attacks by returning large amounts of decoy information to the malicious insiders, keeping this way the privacy of the real users' data.
Density-based methods (Bonneau et al., 2001; Jiang et al., 2003; Shortle et al., 1998; Wang et al., 2004) of decoy selection are taken as an archetypal method, and we consider how enrichment with natives impacts on them.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com