Validation of 18 of the sequences was performed by real-time PCR with primers designed to the manually constructed sequences or Western blot (Method of construction and validation status: Additional file 5).
The 715 m twin tunnel was constructed using the cut and cover method of construction, which involves digging a trench for the tunnel and then roofing it over.
The Design, Novate and Construct (DN&C) system is becoming an increasingly popular method of construction procurement.
But the method of construction is the same.
Each of these hats can be admired for its shape, materials and method of construction.
Although the title announces a sestina, the poem conceals its method of construction.
"It's a way of spanning large areas without columns in the middle, and it's a cheaper method of construction".
The kind of structure was as revolutionary as the method of construction, having more in common with building a bridge than a conventional office block.
This method of construction provides an ingenious substitute for the arch, which was unknown to the Mycenaeans.
At sites where excavations can be made below grade, the trench method of construction may be followed.
The method of construction preserving the permafrost has been called the passive method; alternately, the destroying of permafrost is the active method.
