Traditional methods of concentrating samples by centrifuging and subsequently removing the supernatant are not amenable to the format of microchips.
We tested multiple methods of concentrating the supernatant (rotary evaporation, freeze drying, TCA precipitation) and multiple gel filtration media (Sephadex™ G-50, Sephadex™ LH-20, Superose™ 12, GE Healthcare, UK).
This method uses passive hydrodynamic forces that include inertial focusing and Dean flow, and has a number of advantages over existing methods for concentrating dilute suspensions of microalgae; the method is truly passive, and does not require additional reagents, or external fields or electronics.
This method is an effective means of concentrating impurities, recovering contaminated solvents, cleaning up polluted streams, and desalinizing seawater.
Thermal energy storage has been identified as an effective method for improving the capacity of concentrating solar power plants.
As many more genes than can be sensibly used for downstream applications were identified in the OPLS method, we took the approach of concentrating on transcription factors and of identifying transcriptional modules within our list of candidate genes as a means of selecting a subset of genes that would maximise the information content.
Sensitivity could be increased by using a pre-enrichment step in which the sample is incubated in culture media or a further development of a method to concentrate the bacteria in the specimens before the IFA.
Instead of concentrating on different phases of the method, it was built more as an iterative process.
What distinguishes this method from other types of war-fighting is its focus: instead of concentrating on the enemy you want to kill, concentrate on the civilians you want to protect.
