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For each of the five species tested, we developed a library of DArT clones using the PstI/TaqI method of complexity reduction.
For testing the applicability of PstI/ TaqI method of complexity reduction for the DArT analysis of the rye genome, 12288 clones from a diversity panel constructed from wheat DNA [[ 16], http://www.diversityarrays.com] were printed onto microarrays.
Using the PstI/ TaqI method of complexity reduction we created a rye diversity panel from DNA of 16 rye varieties and 15 rye inbred lines, including parents of a mapping population consisting of 82 recombinant inbred lines.
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The methods of complexity theory can be useful not only in deciding how we can most efficiently expend such resources, but also in helping us to distinguish which effectively decidable problems possess efficient decision methods in the first place.
Different methods of complexity analysis are used to assess changes that have occurred under external forcing in a time series of slips.
Since the structure of the coefficient matrix is Toeplitz matrix, the iteration method with medium-shifting sufficiently employs the fast and superfast direct methods of complexity (mathcal{O}(M^{2})) and (mathcal{O}(Mlog^{2}M)), respectively.
To meet the requirements of a highly complex and at the same time critical system, such as airport security, a standard scenario process has been enhanced by matrix-based methods of complexity management.
Both methods of complexity reduction and procedures for the library construction were performed as reported by Akbari et al. (2006).
An even larger increase in DArT marker density can be achieved by deploying other methods of complexity reduction opening opportunities for rapid identification of tightly linked markers and providing a platform for positional cloning of rye genes.
Initially, the most frequently used seven methods of complexity reduction (all based on a combination of PstI RE and a single 'frequently cutting' RE) were tested in several Brassica accessions by resolving products of representation amplification on 1.2% agarose gel.
The resulting method is of complexity O (n · log (n ) ).
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