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The method of recombinase-mediated cassette exchange (RMCE) enables the efficient generation of an allelic series in embryonic stem cells (ESCs) and mice (Araki et al., 2002).
One proposed method of chemoresistance involves ATP-binding cassette (ABC) transporters found in a subpopulation of cancer stem cells (CSCs), called side population (SP) cells [116].
M1223 SL13444, sipAHA sptPM45) was constructed by allelic exchange of sipA with a gene cassette encoding sipAHA and a kanamycin resistance-gene cassette using the method of Datsenko and Wanner [18] and by chromosomal integration of a suicide plasmid (tetR) encoding the C-terminal region of sptP fused to the M45 epitope (MDRSRDRLPPFETETRIL) [19].
M1269 (SL1344, sipAM45 sptPHA) was constructed by allelic exchange of sipA with a gene cassette encoding sipAM45 and a kanamycin resistance-gene cassette using the method of Datsenko and Wanner [18] and by chromosomal integration of a suicide plasmid (tetR) encoding the C-terminal region of sptP fused to the HA epitope (YPYDVPDYA).
Some children use ribbons of cassette tape.
The manipulator is installed on top of Cassette Multifunctional Mover (CMM) to assist during the cassette removal and installation operations.
Concept drawing of cassette tape.
The development of PCR methods to amplify cassette arrays using primers in the 5′-CS and 3′-CS of Class 1 integrons and the equivalent regions of other MRI and the increase in complete sequencing of resistance plasmids have led to an explosion in the number of cassette array sequences in databases such as GenBank.
Adjacent cassette exons are a pair of cassette exons which are adjacent in the final transcript.
Use the same type of cassette.
In this method the iTol2 cassette, comprising of the minimal cis-sequences of Tol2 in an inverted orientation separated by a ~1 kb spacer, was introduced into a BAC using recombination of homologous sequences within the genomic insert DNA in the bacterial host [ 5].
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