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Szot et al. provide a detailed video account of a rodent islet isolation using a method of bile duct cannulation [ 13].
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They also insist that well-regulated, large farms can offer more humane living conditions and methods of bile extraction.
Despite ongoing reassurances from farmers that methods of bile extraction are "humane" we have seen not one bear to this day who would lay willingly down for such an invasive procedure that compromises their physical and psychological health.
A direct plate assay method was employed for detection of bile salt hydrolase (Bsh) activity.
In this study we have developed a rapid method for the shotgun analysis of bile acids in intestinal fluid.
Here we describe a "shotgun" mass spectrometry method for the rapid profiling of bile acids in ileal fluid.
The grass was soaked overnight in tap water and examined for the presence of infective larvae using the method of [ 18] without addition of bile.
Conclusions: This new method should be useful for the monitoring of levels of bile acid 7α-dehydroxylating bacteria in human fecal samples.
Survival of isolates in the presence of bile was determined according to the method of Vinderola and Reinheimer [ 12].
We believe that this method is a valuable tool to study pathological changes of bile ductules in vitro.
In fact, this effect of bile salts is the basis for a standard method of titering C. difficile spores by colony formation [16].
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