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Therefore, the choice of GNPs fits well for the development of technologically simple, flexible, and cheap method of amplification of the SPR sensor signal.
The apes may employ this method of amplification to deceive the listener into believing they are larger animals.
With its relative ease of implementation and greater sensitivity, WT-Pico is the preferred method of amplification for cases in which sample RNA is limiting.
One method of amplification, PCR based exponential amplification, can generate microgram quantities of cDNA from as little as 1 ng of total RNA.
For nucleic acid detection the processing of the chip will differ only in the technical details required for the specific method of amplification.
This method offers high sensitivity (shown in Figure 4a) and superior specificity to PCR, which enables an inherently nonspecific method of amplification detection, such as an intercalating dye or, in this case, pH detection.
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An interesting alternative is the MALBAC (multiple annealing and looping-based amplification cycles) method of genome amplification, which uses a semi-linear amplification to reduce amplification bias and increase genome coverage [ 15], however this method has yet to be tested on mixed cell populations (i.e. metagenomic samples).
The two factors for the rapid increase are the application of more diverse and intelligent methods for enrichment and isolation and the development of new and ever more sophisticated methods of amplification and sequencing of genes [115].
In these publications, various starting material amounts, methods of analysis, and methods of amplification varied greatly.
However, these methods of amplification can result in misrepresentation of certain genes or genomic regions.
The two methods of amplification were compared to each other four times and twice to themselves in a fully balanced flip dye experimental design including technical repeats.
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