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The obtained data were analyzed according to the ΔΔ Ct method normalizing to human ACTB or mouse Actb mRNA levels.
Transcripts were quantified by the Δ-ΔCt method, normalizing to elongation factor EEF1G.
Fold change in RNA was calculated using the comparative Ct method, normalizing to 18S rRNA or GAPDH control.
Relative cDNA abundance was calculated using the ∆∆CT method normalizing to housekeeper gene expression indicated in the figures.
Relative quantifications were calculated with the 2−ΔΔCt method normalizing to PGK1 or RNU44 for mRNA and miRNA analyses, respectively.
Expression levels were calculated using the comparative C t method, normalizing to the glyceraldehyde-3-phosphate dehydrogenase control.
Similar(48)
(2012) and employed single parameter models (additive normalizing, multiplicative normalizing) to fit our data.
This method involved obtaining CT values for the transcripts of interest normalizing to the housekeeping gene Cyclophilin and comparing the relative differences between control and mutant samples.
Fig. 1 Illustration of similar terms normalizing to a UMLS Concept ID.
A comparison of the three methods of measuring NGAL was conducted by Endre and colleagues [ 19], who showed comparable AuROC values for NGAL to predict outcomes, though favoring normalizing to urinary creatinine.
The results are expressed as relative transcription activity after normalizing to the control empty plasmid.
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