Sentence examples for method normalized to from inspiring English sources

IDO1 mRNA was quantified by Ct method normalized to GAPDH using ABI Prism 7000 software.

In these studies, IFNβ and MxA mRNA levels (as well as all of the cytokine RNA levels) were quantified by the ΔΔCT method, normalized to levels in uninfected control macaques, and reported as fold-change in RNA copies (Figure 3).

Cycling was initiated at 95°C for 10 min, followed by 40 cycles of 95°C for 30 s, 55°C for 30 s and 72°C for 1 min. Samples were run in triplicate and relative expressions of MR and DC-SIGN were calculated using the comparative threshold cycle method normalized to GAPDH (2−ΔΔCT mathematical model [24]).

Results were analyzed by using the Δ-ΔCt method normalized to Actb.

Results were calculated using the 2-ΔΔCT relative quantification method normalized to the HPRT1 gene.

Comparisons of expression levels were determined by delta CT method normalized to β -actin.

Transcripts were quantified by the Δ-ΔCt method, normalizing to elongation factor EEF1G.

Analysis compared expression in shMCU cells to shGFP cells using the 2−ΔΔCt method, normalizing to glyceraldehyde 3-phosphate dehydrogenase expression.

Fold change in RNA was calculated using the comparative Ct method, normalizing to 18S rRNA or GAPDH control.

Relative cDNA abundance was calculated using the ∆∆CT method normalizing to housekeeper gene expression indicated in the figures.

Relative quantifications were calculated with the 2−ΔΔCt method normalizing to PGK1 or RNU44 for mRNA and miRNA analyses, respectively.