Sentence examples for method normalising to from inspiring English sources

The relative expressions of caspase-3, 7, 8, and 9 were calculated using the ΔΔ-Ct method, normalising to β-actin.

RNA expression was calculated using the comparative Ct method normalised to GAPDH.

Expression levels were calculated according to the ΔΔCt method normalised to the Elval1 mRNA expression.

Results were analysed with the Δ-ΔCt method normalised to Actb.

Target gene expression was determined relative to endogenous controls using the comparative cycle threshold method normalised to glyceraldehyde 3-phosphatase dehydrogenase (GAPDH) or β-actin expression.

Gene expression levels were determined using the ΔΔ Ct method, normalised to the housekeeper gene and expressed relative to a calibrator (Winer et al, 1999).

The relative expression level of the collagen-I gene was analysed using the 2−ΔΔCt method normalised to the housekeeping gene GAPDH, which served as an internal control.

Fold changes were calculated by the delta delta Ct method normalised to the four housekeeping genes [ 67] with R (version 2.9.0).

Results were analysed with the delta-delta method normalised to two housekeeping genes (Ppia and Rpl13a or UBC and TBP for mouse and human samples, respectively) and compared with a comparator sample (nonclonogenic luminal (NCL) cells).

The relative quantification of the target gene mRNA used the comparative ΔΔCT-method, normalised to an endogenous reference (GADPH) and a relevant normal control equal to 2−ΔΔCT.

Fold change in ABC transporter expression was determined using the comparative ct method, normalising data to peptidylprolyl isomerase A (PPIA; Lastowska et al, 2007).