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Results were analysed with the Δ-ΔCt method normalised to Actb.
Expression levels were calculated according to the ΔΔCt method normalised to the Elval1 mRNA expression.
RNA expression was calculated using the comparative Ct method normalised to GAPDH.
Gene expression levels were determined using the ΔΔ Ct method, normalised to the housekeeper gene and expressed relative to a calibrator (Winer et al, 1999).
Target gene expression was determined relative to endogenous controls using the comparative cycle threshold method normalised to glyceraldehyde 3-phosphatase dehydrogenase (GAPDH) or β-actin expression.
The relative expression level of the collagen-I gene was analysed using the 2−ΔΔCt method normalised to the housekeeping gene GAPDH, which served as an internal control.
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The relative expressions of caspase-3, 7, 8, and 9 were calculated using the ΔΔ-Ct method, normalising to β-actin.
The relative quantification of the target gene mRNA used the comparative ΔΔCT-method, normalised to an endogenous reference (GADPH) and a relevant normal control equal to 2−ΔΔCT.
Expression values were calculated using the 2-ΔΔct method and normalised to the reference genes GPM1 and TDH3.
The relative quantification was calculated using the ΔΔCt method and normalised to the control group.
RQ values were calculated using the 2− ΔΔCt) method and normalised to an individual naive AnxA1+/+ or AnxA1−/− mouse (calibrator sample).
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