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However, this method needs large volumes of organic solvent, while having low sensitivity.
However this method needs large sample volumes and extensive fractionation.
First, the method needs large sample sizes to work effectively because it uses multiway splits.
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Such rewiring methods need large networks to be interpretable.
Ultracentrifugation followed by RT-PCR amplification has often been used to obtain these sequences, but these methods need large volumes of plasma, and the extended process results in deterioration of the viral material.
However, the Soxhlet method needs a large amount of solvent and it is time-consuming.
When calculating the reliability of aerospace mechanism with too small failure probability, the Monte Carlo simulation method needs too large sample size.
The former method needs a large amount of organic solvent (consisting of light liquid paraffin and heavy liquid paraffin) to serve as continuous oil phase [15], and the latter have poor mechanical strength because of weak ionic bond formed through an electrostatic attraction between chitosan and sodium triphosphate (STPP) [16].
However, the method needs a large amount of computational resources in terms of both memory space and CPU time.
The Elixhauser method has more variables than the Charlson based methods, and therefore needs larger sample size.
This method needs to solve a large number of optimization programs and select the most feasible solution by additional criteria.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com