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However, the PIM method needs a sacrificial binder, which increases working procedure and cost.
However, the method needs a higher skill in logical thinking during the process, which demands time.
However, the ICP method needs a good initial parameter to obtain the global optimum transformation matrix, which is difficult to guarantee in the actual inspection process.
However, the Soxhlet method needs a large amount of solvent and it is time-consuming.
Their method needs a part of complete data vectors to pre-train a SOM and then impute incomplete data.
In fact, Li et al. method needs a previous pre-classification to discern between non-crack and crack images.
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This method needs an extension over the crack initiation period.
The development of a quantitative method needs an optimization of both detection and derivatization step.
The method needs an initial calibration phase based on multi attribute decision making-simple additive weighting (MADM-SAW).
The extracellular method is more advantageous than intracellular method because the intracellular method needs an additional step to obtain the purified nanoparticles[5, 6].
Last but not least, the identification of the microarray results does not need any special instrument, whereas the ELISA method needs an enzyme immune analyzer, and other fluorescent immunological tests need an expensive fluorescent scanner or detector.
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