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The current medfly weekly monitoring method, manual counting, results in a suboptimal spraying frequency in citrus orchards.
Control Method: Manual, mechanical and chemical control methods are all effective; employing a combination of methods often yields the best results and may reduce potential impacts to native plants, animals and people.
The semiautomatic method was compared with the usual manual method (manual contour tracing) in terms of its ability to measure a known volume of water (gold standard) as well as the volume of 29 bone cysts.
Table 2 Average CPU time of methods Method Manual segmentation Watershed PolarSnake GaborFM Average CPU time (s) About 80 19.98045.49 10.80.
Two indices were calculated from each patient's PET/CT scan: one based upon the manual method (manual PET index) and one based upon the automated method using the SUV threshold of 15 (automated PET15 index).
Non-binary approaches allow a more detailed way of describing the current state of an indicator but requires a method (manual or automatic) that specifies how to set the indicator state based on available evidence.
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Preparation of templated DNA Capture beads for next-generation sequencing followed the emPCR Method Manual-Lib-L-LV (Roche Applied Science).
A similar method, 'manual programmed' allows the operator to program a specific rate and magnitude of current variations, making it useful for specialized applications.
The bisulfite amplicon libraries were diluted, pooled and clonally amplified in an emulsion PCR (emPCR) and sequenced on the Roche/454 GS junior system according to the manufacturer's protocol (Roche emPCR Amplification Method Manual Lib-A and Roche Sequencing Manual Lib-Al).
For sequencing on the Roche 454 Genome FLX sequencer, DNA libraries were constructed using the Roche shotgun library preparation method for low molecular weight DNA without prior nebulisation (GS FLX General Library Preparation Method Manual, Roche).
The Roche A and B adapters with a MID (Multiplex IDentifier), were ligated to each purified pool of amplicons according to the Shotgun protocol for Low Molecular Weight (LMW) samples (Manual: 'GS FLX Shotgun DNA Library Preparation Method Manual' (December 2007), skipping the fragmentation step.
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