As far as the authors know, this method was started with the pioneering paper of Lazer and Solimini [17].
The development of the super-pressure balloon using this method was started from a 3-m balloon with a polyethylene film covered by a net using Kevlar ropes.
These methods were started to be very basic and simple like the 1/3 rule.
The qPCR methods are started to be more frequently used for environmental investigations and monitoring of the most common fungal contaminants present inside buildings (Bellanger et al. 2009; Roussel et al. 2013), such as performed by the US Environmental Protection Agency EPAA).
The old-fashioned and dishonest methods are starting to die away.
Agile methods were starting to gain strength both in the academia and in the industry.
Developments in miniaturisation and quantitative amplification methods are starting to generate more quantitative and qualitative data from single cells.
Computer aided design methods are starting to gain acceptance in the field of life sciences and drug discovery, thus giving rise to a discipline coined Computer Aided Drug Discovery CADDD).
While early research in the field has been restricted by the limited availability of micro- and nanofabrication techniques, new approaches involving the use of advanced fabrication and surface immobilisation methods are starting to emerge.
