This method was rapidly superseded by approaches using NGS techniques, for example pyrosequencing was initially used to sequence all the RNA (both rRNA and mRNA) from soil communities (Leininger et al. 2006; Urich et al. 2008), while subsequent approaches combined rRNA subtraction with NGS in marine environments (Frias-Lopez et al. 2008; Gilbert et al. 2008, 2010c).
With aging population, increase in longevity and decrease in the number of qualified donors, the need to find alternative solutions to current organ replacement methods is rapidly becoming a critical issue.
However, the improvement of performance with these methods is rapidly becoming impossible due to physical and lithographic constraints.
Although the use of some recently developed marker techniques in plant sciences is not yet as extensive as that of well established methods such as SSR markers, the number of studies utilizing these advanced methods is rapidly increasing [ 5].
Although the number of LC-MS/MS methods is rapidly increasing [ 4– 7], gas chromatography (GC -mass spectrometry (MS)/(MS) is still the standard routine analysis instrument in many forensic laboratories [ 4, 8– 10].
The treatment of gases using biological methods is rapidly gaining acceptance as a sustainable alternative to more conventional techniques such as chemical scrubbing, incineration, and adsorption where a pollutant is only transferred from the gas to other phase (adsorption) or where toxic by-products can be generated (chemical scrubbing and incineration).
The clothing and shoe industries, initiated in the 1860s, were stimulated by demands of the American Civil War, and mass production methods were rapidly developed.
Numerical methods are rapidly gaining popularity for hydroacoustic applications.
Methods are rapidly evolving to extrapolate data at an aggregated level that also remain beneficial to epidemiology, and technology is increasingly central to this the emergent area called digital epidemiology.
Geospatial methods are rapidly emerging as an accessible tool for injury researchers to better understand complex injury aetiology but to date, few authors have made use of their full potential in the major injury cause categories.
