A high pH LC/MS/MS method was optimised for rapid and sensitive analysis of both analytes in rat, dog and human plasma.
A microwave-assisted extraction (MAE) method was optimised for extracting 2,4,6-trichloroanisole (TCA), 2,3,4,6-tetrachloroanisole (TeCA), pentachloroanisole (PCA), 2,4,6-trichlorophenol (TCP), 2,3,4,6-tetrachlorophenol (TeCP) and pentachlorophenol (PCP) from oak barrels.
The method was optimised for samples containing 100% maize of which 0.4 0.6% was of GMO origin.
RNA isolation methods were optimised for high sensitivity, using only 3 μg of total RNA that were reverse transcribed using random hexamers, with the resulting cDNA hybridised after labelling to an oligonucleotide array.
The method was optimised and evaluated for sample preparation, instrument analysis and method performance.
Although the method outlined here is optimised for scoring common carp mitochondrial haplotypes using the Rotorgene 6000 machine, real-time PCR and HRMC analysis can be applied in a similar way to almost any species and/or loci, with a number of different real-time PCR machines available for scoring genetic differences.
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