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The dominant analysis technique for untargeted metabolomics is chromatography coupled with mass spectrometry (MS) but this method is hindered by a large number of unknown peaks [5] and the limited number of reference spectra available to identify the peaks [6].
The failure of in situ hybridization in detecting these sequences can result not only from that detection of the DNA sequences shorter than 5000 bp by this method is hindered, but also with the divergence between the probe used for hybridization and the homologous sequences within the studied species.
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However, the traditional in-cell NMR method was hindered by low signal sensitivity and complicated resonance assignment of proteins in vivo (Hansel et al., 2014).
Conventional methods, such as the pendant drop method, are hindered by large fluid volumes that require several hours to thermally equilibrate between measurements.
Numerical simulations of such flows using the standard direct simulation Monte Carlo (DSMC) method are hindered by high statistical noise, which has motivated the development of several alternate Monte Carlo methods for low Mach number flows.
Real data axial positioning, Figure 11B, indicates unresolved dipole positions at or beyond the arbitrary axial limit of our calculated emission pattern suggesting that either the human judged focus on the single molecule sample is incorrect or that the fitting method was hindered by practical uncertainties like a spatially uneven background.
The application of these methods is hindered due to their high cost.
The usage of theoretical methods is hindered due to considerable variability of tool and process material characteristics even within one delivery lot.
However, the efficiency of dry treatment methods is hindered by the limited final conversion of the solid reactant, due to an abrupt decline of its reactivity during the reaction process.
In recent years, computer aided redesigning methods based on genome-scale metabolic network models (GEMs) have played important roles in metabolic engineering studies; however, most of these methods are hindered by intractable computing times.
As a result of this fact uncertainty analysis in nonlinear inverse problems has been approached in a probabilistic framework (Bayesian approach), but these methods are hindered by the curse of dimensionality and by the high computational cost needed to solve the corresponding forward problems.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com