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The method interrogates Raman shifts originating from molecular vibrational energy levels, and therefore, allowing to distinguish structurally similar molecules if they have distinct vibrational spectra.
Although [F]-fluoro-dopa PET is a perfectly valid and useful functional marker of dopaminergic activity, this method interrogates the ability of dopamine neurons to synthesize dopamine, and it may indirectly measure the dopamine-releasing capacity of dopamine neurons.
Inspired by previous sequence-based computational prediction algorithms [Ng and Henikoff, 2001; Thomas et al., 2003], our unweighted/species-independent method interrogates sequence conservation through the underlying amino acid probabilities modeled by the internal match states of several HMMs representing the alignment of homologous sequences and conserved protein domains.
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Optimal pH conditions were determined using a design of experiments method interrogating buffer strength, CO2% and device preparation conditions.
As applied in this study, each method interrogated different, but overlapping, portions of the methylome.
Further, since some of these methods interrogate multiple populations to enhance their power to detect sweeps2,4,5, the interpretation of the results will be dependent on the set of examined populations, which complicates the assessment of selection pressures in a single population of interest.
Indeed, quantifying or measuring this kind of biological effect might easily differ depending upon the method used to assess hypomethylation (e.g., some methods interrogate the important small RNA sequences, whereas others do not), consistent with the current literature.
The use of multiple methods, interrogated under KMR, not only added to the robustness and trustworthiness of the data but more importantly these methods were found to be culturally acceptable by the Māori participants.
Here, we describe DNA affinity purification sequencing (DAP-seq), a high-throughput TF binding site discovery method that interrogates genomic DNA with in-vitro-expressed TFs.
Here we report the development of the Golgi two-hybrid (G2H), a method that interrogates protein interactions within the Golgi, where transcriptional activators can be assayed with negligible background.
The Finnish method additionally interrogates the resonance resulting from the multiple methylene groups found on these lipids [33].
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