While the docking simulations correctly predicted binding trends, limitations in the method, including potentially inaccurate scoring functions, the use of rigid proteins, and a lack of solvation could have contributed to its inability to reproduce the large differences observed in the IC50 values.
Although the implementation of predictive methods could be of more interest for the European countries, the operational approach of the method includes some practical matters potentially of interest independently from the specific country or region.
These methods need to include potentially cooccurring stressors associated with effluent discharge, such as conventional pollutants, local in-stream habitat alterations, and hydrological and chemical perturbations caused by changes in land-use and land-cover (e.g., urbanization).
However, there are a number of additional theoretical considerations that need to be mentioned, as the wCCS method currently does not include potentially important information.
An alternative method would be to include potentially prevalent cases but assume that there is an unknown fraction of patients who are already in the preclinical stage at baseline (Yen and Chen, 2007).
(Including potentially dangerous wheelchair users and schoolchildren).
There will be other issues, including potentially large budgetary impacts.
Including, potentially, my family.
With the additional "forceps squeezing" method included, the revised version is potentially much improved.
Well-tried and potentially effective methods include visiting door-to-door and calling by telephone (landline or mobile), both usually done without prior warning (cold-calling).
