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Furthermore, pinpoint modification of endogenous plant genes is also an attractive method in molecular plant breeding.
In this section, we review the most commonly used amplification method in molecular laboratories worldwide, the polymerase chain reaction (PCR).
RNA interference (RNAi) is an essential method in molecular biology to reduce the expression of target genes and thereby determine their function.
Reverse transcriptase is an indispensable enzyme for real-time reverse transcriptase (RT -PCR, a standaRT -PCRod in molecular diagnostandardr detection and quantification of defined RNA methodles.
Phylogenetic analyses were conducted using the Maximum Composite Likelihood method in Molecular Evolutionary Genetics Analysis MEGAA) software version 4.0.2 [74] and bootstrap consensus trees were inferred from 1,000 replicates.
Thus, site-directed mutagenesis is a central method in molecular biology.
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