One of the most important diagnostic methods in heart and vessels diseases is echocardiography, which enables to examine morphological and physiological disorders.
The target compounds were tested for their in vitro antibacterial activity against those two challenging organisms by the microdilution method in brain heart infusion broth.
As described subsequently, we have verified all findings from NIC recording with independent methods, specifically via an optical method in intact hearts to follow fluid phase uptake of fluorescent probes and via patch clamp of isolated myocytes.
We estimated survival rates, cardiac function by echocardiography, blood pressure measured by indirect tail-cuff method, telomere length in heart tissue and white blood cell were evaluated by quantitative fluorescence in situ hybridization (Q-FISH) analysis.
Furthermore, the method could help, in heart tissue engineering and bioartificial heart researches, to "engineer the heart piece by piece" [ 23].
As there is no clinical experience in patients with denervated hearts, we investigated this monitoring method in patients after heart transplantation.
However, the presence and activity of NOS and neuropeptides which may modulate autonomic nervous transmission in fish [ 35] need to be examined with more direct methods in the heart of crucian carp.
Inhibition of ROS by over-expression of antioxidant enzymes using transgenic methods in the heart reversed diabetic cardiomyopathy in animal models of both type 1 and type 2 diabetes [ 3, 13, 23- 25].
Methods of decellularisation in heart valves report the use of trypsin (Bader et al. 2000) or a non-ionic detergent like triton X-100 followed by ribonuclease digestion (Bader et al. 1998) which leads to incomplete removal of cells.
The new method for delineation of the LV, EXINI heart (ExH) software (Exini Diagnostics, Lund, Sweden), was based on a previously described method in which a heart-shaped LV model and an active shape algorithm are used instead of geometrical approximations such as ellipsoid or hybrid cylindrical/spherical models [ 18].
