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Through the design of an experimental case scenario, we highlight the considerations and potential limitations of this method in forensic casework.
With the technical advances of NGS technology and continuous translational efforts of forensic scientists, we believe that NGS technology is likely to become an easily accessible routine method in forensic practice.
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DNA-based methods in forensic entomology have advanced rapidly since the first successful attempt to discriminate important forensic insect species in 1994 [ 1].
However, the direct application of these methods in forensic analysis has to be made even more cautiously than for general scientific enquiries given the potentially serious consequences of a wrong inference or conclusion in a criminal setting.
Validation parameters LOD, LOQ, linearity, recovery, accuracy and imprecision of the assay were studied according to international method validation guidelines in forensic toxicology (SOFT/AAFS, SWGTOX).
The SALLE based protocol provided as good results as the conventional extraction method (ultrasonic assisted extraction followed by solid phase extraction, UAE SPE) and hence the method can be applicable in forensic and biomedical sectors.
This paper highlights the limitation of the methods used in forensics compared with a CBR method in the analysis of forensic evidences.
Despite advances in forensic methods, the full magnitude and significance of POPs in the aquatic environment is largely unknown.
Considerable efforts are being extended to develop more effective methods to detect drugs in forensic science for applications such as preventing doping in sport.
Testing for poor-quality medicines is challenging because of the high cost of traditional laboratory-based analytic chemistry methods, lack of training in forensic techniques for packaging and chemical analysis, and the large sample sizes needed to conduct representative and generalizable studies in the field.
This is the first DLLME-HPLC-PDA method which optimized for postmortem urine samples and should be considered as an applied analytical method for determination of opioids in forensic toxicology laboratory.
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