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In addition, the described procedure must be performed by medical professionals, and this is the biggest drawback to the method in endemic settings.
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Even though one may screen other enzyme combinations to increase polymorphism, our results suggest that radioactive AFLP is not an easy method for strain differentiation in endemic regions.
On the other hand, the RT methods are more aggressive in endemic areas than in other areas [ 1, 10].
This may be a useful method for detecting malaria parasites in endemic areas.
Although assessment of the environmental load of B. pseudomallei is important for risk assessment in humans or animals in endemic areas, traditional methods of bacterial culture for isolation have low sensitivities and are labor-intensive.
The PPR disease control strategy in Somalia was initially though country-wide vaccination campaigns, the method prescribed for the control of PPR in endemic countries (Diallo 2004).
This method could be applied in resource limited settings, for surveillance in endemic regions or for routine epidemiological screening.
A literature search was conducted to identify cross-sectional data on the parasite densities of individuals with Plasmodium falciparum infections in endemic areas measured using a quantitative molecular method.
The recently developed loop-mediated isothermal amplification (LAMP) method is relatively simple and can be improved for better use in endemic countries.
Furthermore, serological methods have low discriminatory power differentiating latent from active TB infections in endemic regions [9].
There is a need for methods to accurately diagnose and discriminate between latent and active TB infections in endemic regions.
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