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The most extensively studied method in cell immobilization is entrapment.
Cellular membranes were lysed by a freeze thaw method in cell lysis solution (0.2% Triton X-100, 10 mM Tris (pH 7.0), and 1 mM EDTA).
The biotin avidin system quickly became a central method in cell biology, immunology, and protein engineering, as well as electron microscopy.
It has become clear that there is no single 'best method' in cell tracking.
In a genome wide screen performed by radiation hybrid method in cell lines, larger numbers, namely 2500 genetic interactors of VAP and VAPA have been identified.
Figure 3 shows the highly weighted motifs obtained from our method in cell cycle-related gene set and non cell cycle set, and the relative positions of those motifs are presented in the weight distribution of all motifs.
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The numerical advantages of the diffusing-vortex scheme over other conventional vortex methods, Cloud-in-Cell methods, particle methods, and some finite difference schemes are evaluated in terms of reducing total CPU time, avoiding cutoff procedures, and sidestepping various interpolations.
When combined with new technical methods in cell processing and culturing, the therapeutic potential of T cells manufactured in academic centers has improved dramatically.
Some molecular approaches to cell biology using D. discoideum are detailed in Methods in Cell Biology.
Metabolic events were detected according to the protocols from Methods in Cell Biology.
To minimise delivery losses using the long cannula we tested several vector delivery methods in cell culture.
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