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We additionally employed the cold trypsin method; however we found that cold trypsinisation removed a substantial amount of epithelial cells.
We did not implement a brute-force volume update method, however we would expect the overall algorithm described here would be much faster by comparison.
Other, small improvements which we could have made to our program were to add a winning song or a scoring method, however, we decided that these features were not necessary for our program.
For minimizing the discrepancy functional we use the adjoint problem method; however, we use it not only in iterative regularization, but in Tikhonov and parametric ones as well.
In our proposed method, however, we can simultaneously use multishot data in estimating local attenuation coefficients because the initial source amplitude is canceled out in generating the amplitude ratio gather with Eq. 3, and the amplitude ratio gather does not depend on the source amplitude or source location.
With our 1D-gel method, however, we analyzed the whole gel lane and did, thus, not rely on visible protein staining.
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Unlike previous methods, however, we apply these coupled interactions implicitly by adding the constraint to the pressure system and combining it with any implicit solid forces in order to obtain a strongly coupled, symmetric indefinite system (similar to [17], which only handles incompressible flow).
It is out of the scope of this paper to review the nuances of age and uncertainty calculations for specific dating methods (Walker (2005); Taylor and Bar-Yosef (2014) provide recent overviews of quaternary dating methods); however, we briefly discuss the chronological metadata included in the database in the 'Chronological metadata' section.
Based on the different underlying references for the various methods, however, we could hypothesize that similar methods (e.g. two manual parcellations of the same brain) would give highest overall concordance, whereas methods that relied on different references and/or registration schemes would likely have lower degrees of similarity.
It is possible that increasing the incubation time would improve the FAME-recovery for the BF3-method; however, we decided against proceeding with this catalyst, since it is hazardous to work with, expensive, and its shelf life is limited [ 20].
Among the three methods, however, we recommend to estimate and S using the summary statistics in Scenario.
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