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Although the delta-delta Ct method has stricter requirements, it can substantially reduce the number of PCR reactions.
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Mathematical programming methods have strict requirements on the model itself (e.g. the problem or the continuous relaxation of the problem should be convex) and can provide more clues on the quality of the final solution [22].
Parametric methods have strict assumptions on the analyzed data, including: normal distribution, homogeneous variances between data groups and continuous measures with equal intervals.
In our work, a strict method has been applied, the neighbor-jackknife test.
Given this strict assumption, the method has been successfully applied in time series analysis recently, whereby the proxy outcome was described as a control series [ 23, 24].
Brazil has strict campaign-finance laws.
Still, their own game has strict rules.
"Surkov has strict criteria," Mr. Dorenko said.
Pakistan has strict blasphemy laws.
Especially if your camp has strict rules.
California has strict regulations on battery disposal.
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