Hitherto, these methods have required manual measurement by highly skilled human observers.
They have relied on sampling populations that have been identified beforehand because most population genetics methods have required discrete populations.
After nearly 20 years with a more or less unchanged design, declining participation rates by certain person groups and new survey methods have required fundamental changes in the survey design.
However, most methods have required LIF and culture vessels coated with animal derived materials, such as collagen, gelatin and complex matrix [7], [8].
Although we have used a number of bioinformatic methods to identify and align aspartic protease sequences, these methods have required manual supervision to construct reliable phylogenies.
To date, these techniques and other modern methods have required conventional brightfield microscopy, involving the unwanted capture of out-of-focus light, and time-consuming reconstruction of serial histological sections or optical image planes.
To date, this has required methods that are both complicated and time-consuming, resulting in limited application and the potential for analysis errors due to poor understanding.
To date, this has required methods that are complicated and often time-consuming (state-space models), resulting in limited application of this technique and the potential for analysis errors due to poor understanding of the fundamental framework behind the approach.
Measurements were made manually in preference to automated methods, because any automated method would have required validation for this patient population.
That transport method would have required many people, and led to deforestation and environmental ruin that would've caused the population to plummet.
