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This method has existed for a while, but few have dared use it (without explicit permission) in the past.
Architecture education with apprenticeship based training method has existed from long time ago.
Until now, no method has existed to change PIP2 in a known, quantitative and controlled manner in membranes in which GIRK channel activity is measured.
Although microarray data inadvertently overcame this bias through the manual adjustment of laser intensities during microarray scanning, until now no method has existed for correctly normalizing DamID-seq datasets.
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Up to now, only a few suitable tools and methods have existed for farmers and regional stakeholders that support the process of decision-making in this field.
This is in stark contrast to fluid-phase systems, for which accurate scale-up and design methods have existed for decades.
As has been reviewed recently [18],[19], the use of phytomass as a substrate for generating vermicompost had been very limited prior to the introduction of this know-how and no methods had existed on vermicomposting of phytomass which had the potential for large-scale utilization.
Two traditional methods have existed since Peking opera first appeared in China.
In addition, while it may be of interest to look at divergence on specific axes in some studies, morphometric methods have existed for several years that allow for tests of differences in means and variance in a wholly unconstrained shape space [3], [13], [14].
Although the methods have existed for many years, they are underutilized and provide a rich data set that is ideal for pharmacokinetic modeling.
Indeed, these methods have existed long before GS emerged (Ritland 1996, 2002; Nejati-Javaremi et al. 1997; Lynch and Ritland 1999; Eding and Meuwissen 2001).
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