Results show that the new group contribution method gives low deviations and can be used with confidence in thermodynamic and engineering calculations.
The proposed method gives low false-positives due to the integration of different features.
This observation, together with the fact that filtered data with the MAS5-Spearman method gives low coverage (as indicated above the total amount of positive pairs was only 2,198), brings us to the resolution of not using the filter for MAS5 dataset.
Nevertheless, by increasing tilted angle above 60°, the difference between the two methods is very significant and GA method gives lowest cost.
There was a clear correlation between the absorbed dose determined by the simpler PA method and the more labor-demanding CV method, but the PA method gave too low values of the kidney dose.
We found a clear correlation between the values obtained with the two methods, but the linear regression coefficient was far from unity, demonstrating that the PA method gave too low D/A administered values.
We optimized reaction conditions of each method, that is, reaction buffers, temperature, molar ratios of [mRNA]:[splint]:[Linker-S], etc. Comparisons of the results of the three puromycin-linker attachment methods are shown in Fig. 2. The DNA ligase method gave a low yield.
Finally, at the mobile phase composition described under the 'Methods' section, the method gave the lowest peak tailing factor and the highest theoretical plate count.
Notably, sonication or sheering followed by salt extraction gave low APE1 activity yields, whereas the other three methods gave high yields that were comparable with each other.
Interaction with filament binding proteins is however difficult to probe with our method, given the low concentrations of mutant actins produced in reticulocyte lysates.
