Sentence examples for method from triplicate from inspiring English sources

Expression was determined by the ΔΔCT method from triplicate reactions.

To demonstrate the applicability of the FTA® Elute method for the analysis of surface samples, DNA was extracted with this method from triplicate swab samples taken from the interior of the International Space Station (ISS) as part of the "SAMPLE" experiments, which were previously described by van Tongeren et al. in 2007 [ 2].

PCR efficiencies were calculated by the standard curve best fit method from a triplicate dilution series experiment for each gene and cDNA sample (Pro-Pper/Amastigotes).

The reproducibility of the method was determined from triplicate kinase assays of 50 nM CaMKII in the presence of the cofactors calcium and calmodulin on protein microarrays containing 3019 different human proteins printed in duplicate and a total of 6244 accessory features (control proteins and buffer/no protein spots).

Total genomic DNA was extracted from triplicate samples using the method described by Westerholm et al. [26].

RNA was extracted from triplicate cultures using the hot-phenol method and subsequently cleaned up further on columns with the QiaGen RNA purification Kit.

RNA was extracted from triplicate cultures of each sample using the method by Chang [ 46] with some modifications.

For all qRT-PCR analyses, data from triplicate reactions were analyzed using the 2-ΔΔCt method.

Primers are detailed in table 1. Ct values were obtained from triplicate fermentations and were normalized using the 2-ΔΔCt method (Wong and Medrano 2005).

Representative expression levels (from triplicate experiments) at passages 1, 5 and 8 were used to determine the effect of the culture method on the pluripotency of the cells.

Results are mean values from triplicate cultures.