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The genomic DNA was extracted using the Phenol/Chloroform method from peripheral venous blood.
DNA was extracted by standard phenol-chloroform method from peripheral blood.
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Human PBMCs were prepared by specific gravity centrifugal methods from peripheral blood.
Total genomic DNA was extracted by standard methods from peripheral blood lymphocytes or muscle biopsies.
Total genomic DNA was extracted by standard methods from peripheral blood lymphocytes.
Genomic DNA was extracted by conventional methods from peripheral blood, and whole-genome copy number variation (CNV) analysis was performed with the Affymetrix Genome-Wide HumArrayP Array 6.0 (Affymetrix, Santa Clara, CA, USA).
The metaphase chromosome spreads were obtained by standard methods from peripheral blood lymphocytes of normal human (HSA) donors, and from the primate lymphoblastoid or fibroblast cell lines Gorilla gorilla (GGO) and Pan troglodytes (PTR).
24 DNA was extracted using standard phenol-chloroform or salting-out methods from a peripheral whole blood sample obtained from all cases.
Genomic DNA was extracted by a standard phenol/chloroform method from leucocytes in peripheral blood.
Mean leukocyte telomere length was derived from the mean of the terminal restriction fragment length by using the Southern blot method on DNA extracted from peripheral leukocytes, as described elsewhere (5).
Karyotyping with G-banding was performed using standard methods on lymphocytes obtained from peripheral blood and fibroblasts from a biopsy of skin with or without hypochromic cutaneous lesions.
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