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DNA was extracted by the standard phenol/chloroform method from frozen tissues.
DNA was extracted via a standard phenol chloroform method from frozen skeletal muscle tissue (CWD-negative white-tailed deer), frozen retropharyngeal lymph nodes (matched case control white-tailed deer), and ethanol-fixed tissues (matched case control mule deer) all stored at −20°C.
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In order to further increase the throughput of molecular analyses, we are developing methods to print tissue lysates from frozen samples in an array format.
Here, we describe methods for the purification of αSyn from frozen human cortex.
Genomic DNA was prepared from frozen tissues by standard methods previously described [ 5, 12].
Genomic DNA was extracted from frozen rice leaves by CTAB method as described by Doyle and Doyle (1987).
Genomic DNAs of the F2 plants as well as the parental plants were extracted from frozen leaves using the CTAB method.
Total RNA was extracted from frozen tissue using the TRISOL method (Sigma).
Later, Genomic DNA was extracted directly from frozen specimens using standard phenol/chloroform method after proteinase K digestion.
Thus Benzonase accessibility is associated with euchromatic features, demonstrating that the TACh method identifies accessible regulatory regions of the genome from frozen tissue.
Total RNA was isolated from frozen samples using the lithium chloride method.
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